CLS
2521 LABORATORY for HEMATOLOGY I
Fall Semester 2008
1:00 p.m. - 2:50 p.m.
1:00 p.m. - 4:50 p.m.
The laboratory class meets in CHI 115
COURSE DESCRIPTION
Performance of laboratory procedures necessary to function in a clinical
hematology laboratory. Prerequisites: CLS 1512 Principles of
Clinical Laboratory Science and CLS 1511 Laboratory for Principles of Clinical
Laboratory Science. Corequisite: CLS 2523 Hematology I.
SYLLABUS
DATE
Laboratory
Activities
AUG 25 (M)
Review/Kodachromes
27 (W) WBC Pipets/ Hemocytometer
SEP 01 (M) ☺
◊ ☺ ◊ ☺ Labor Day Holiday ☺ ◊ ☺ ◊ ☺
03 (W)
WBC Pipet/Hemocytometer
08 (M) Kodachromes
10 (W)
Unopettes /Hemocytometer
15 (M) “Diff’s”/Kodachromes
12 (W)
Unopettes /Hemocytometer
22 (M) Monday’s
labs devoted to blood smear evaluations (diff’s)
24 (W)
Introduction to Wright’s staining techniques, practice making
blood smears. Students assigned to turn in one near perfect
slides and nine very good slides.
29 (M) Blood Smear Evaluation
(continued)
OCT 01 (W) Preparing
blood smears (from previous Wednesday).
06 (M) Blood Smear
Evaluation (continued)
08 (W) LAP Stain
13 (M)
Blood Smear Evaluation (continued)
15 (W) The Permanent Slide,
Reticulocyte count
20 (M) Blood
Smear Evaluation (continued)
22 (W)
Osmotic fragility,
27 (M) Blood Smear Evaluation
(continued)
29 (W)
Manual Microhematocrit, preparing the buffy coat slide,
and staining.
NOV 03 (M) Blood Smear
Evaluation (continued)
05 (W) Hemostasis lab
10 (M)
Blood Smear Evaluation (continued)
12 (W)
Special Techniques: ESR(Westergren and Wintrobe), Plasma
Recalcification Time, Clot Retraction.
17 (M) Blood Smear Evaluation
(continued)
19 (W) Hemostasis Lab
24 (M)
Blood Smear Evaluation (continued)
26 (W) ☺ ◊ ☺ ◊ ☺
Fall Break / Thanksgiving Holiday ☺ ◊ ☺ ◊ ☺
DEC 01 (M) Blood Smear
Evaluation (continued)
02 (W) Final
Lab Exam
08 (M)
Final Week begins.
About the lab activities..... Monday lab sessions will be dedicated to
completing the assigned stained blood film case studies (referred to as ‘diffs”).
You will need to average three “diffs” per week. In order to take advantage of
the bonus points (explained in grading and evaluation strategies), you will have
to come in at times other than the designated lab time to complete this
assignment. The instructor also reserves the option to change lab activities. If
such changes are made, they will be announced in advanced and lab procedure
provided.
TEXTBOOKS
01. Required. Rodak, B. F., G. A.
Fritsma, K. Doig. (2007), Hematology:
Clinical Principles and
Applications. 3rd Edition. Saunders
- Elsevier.
02. Required. Abbott Laboratories' Medical
Atlas. The Morphology of Human
Blood Cells.
This may
purchased at the book store or you may order from:
ADD Distribution Center
c/o COMAC, INC
Attn: Medical Atlases
1521 East Wilson
Batavia, IL 60510
To order, the cost is $12.00 and will take up to four weeks for delivery.
Make your check payable to Abbott Laboratories. The atlas cannot be
returned.
03. Required. Jarreau, Patsy; Clinical
Laboratory Science Review: A Bottom
Line Approach. 3rd
Edition. 2005
04. Optional. O’Connor, Barbara; A Color Atlas
and Instructional Manual of
Peripheral Blood Cell Morphology. Williams and
Wilkins. Optional
05. Class notes for CLS 2523
(these will be available electronically through
Blackboard 6).
NOTE
If you have a disability or need special
accommodations, it is your responsibility to notify the instructor.
GRADING AND EVALUATION STRATEGIES
01 The Monday laboratory session is
generally reserved for performing stained
blood films. The
Wednesday laboratory generally reserved for laboratory
projects. The first
laboratory project assignment will be a review of the
hematocytometer and its
use.
01 One major lab exam (100 points). This will be given
at the end of
the semester.
02 40 Case Studies (stained blood film evaluations) to be
completed over the
semester. Each case study is 20
points (with a maximum total of 800 points).
A. In order
to complete this assignment in a timely manner, you must
turn in a minimum of three completed case studies each week.
B. A 50 point
bonus will be awarded to those students who complete
their 40 case studies and turn them in the week before the fall break.
C. All case
study reports must be completed (front and back) using the
required forms which will be supplied by the instructor or may be
down loaded from Bb.
04 All lab assignments (completed on Wednesdays) will be
recorded and
turned in for a grade.
Points will be awarded and will range from 5 to 20
points dependent upon the
complexity of the lab activity.
05 Affective Domain grade (100 points). See Affective
Domain information
located in Bb6 and the
CLS and CLT Student Handbook.
06 Optional. Up to 5 lab exams may be given during the
semester. Each lab
exam and what it will
cover will be announced one week in advance and
will be given at the beginning
of the lab session. Each exam will have a time
limit. A maximum of 25 points
will be assigned to each exam.
07 See the syllabus for CLS 2523 for other applicable
grading and evaluation
strategies.
NOTE
If you have concerns about the grading of any of the exams, quizzes, or other
graded materials in lecture or laboratory; you must address these concerns
within five classroom days following the return of such materials. If you do not
act to express concern, this is your approval of the matter of grade(s) and the grade(s) will stand.
ATTENDANCE, LABORATORY PARTICIPATION, AND
LABORATORY ASSIGNMENTS
01 The same attendance rules apply as given in the syllabus for
CLS 2523
Hematology I.
02 Attendance will be taken in each lab session. An
attendance sheet will be
provided for your signature.
Sign in only for yourself.
03 The Monday laboratory sessions will have one hour for the
first four weeks
set aside for Kodachrome
review. The second hour will be given to working
on the stained blood smears.
A. Go to Blackboard 6, the 'Assignments' section and CLICK on
the URL
address for the instructions
for examining stained blood films.
B. This
sectIon contains the instructions and forms need to conduct a
stained
blood film evaluation.
C. If you
would like to have additional help and instruction regarding the
recognition of blood cell morphology, ask the instructor or lab associate.
04 The Wednesday laboratory sessions are set aside to provide
instruction in
skill development and testing
methodology in the hematology laboratory.
The daily assignments may be
seen in the schedule at the beginning of this
syllabus.
NOTE: Some of the
laboratory procedures and activities set up
for the Wednesday laboratory
activities may be of short duration and in
which case, use the remaining
time to work on the stained blood films.
A.
Wednesday (25th) lab: Hemocytometer activity. Go to Bb6 and
open
up to the assignment's section. Click on the first URL address.....Lab
activities hemocytometer. Copy and read the instructions.
B.
Wednesday (September 3rd) lab: Hemocytometer activity. Click on
the second
URL address..... Use hemocytometer pipets. Also click on
the third
URL address..... Thoma pipets and math. This unit is an
extra lab
exercise that will be explained in lab. This lab procedure will
repeated the following week (Wednesday, 6th). Copy and read the
instructions.
C.
Wednesday (5th): Continuation of the previous week.
D.
Wednesday (12th): Hemocytometer and Unopettes. Click on the
fourth URL. Copy and read the instructions.
E.
Wednesday (19th): Preparing and staining blood films. Go to Bb6
and click on the URL address for preparing and staining blood
films. Copy and read the instructions. This activity
will cover two to
three lab sessions.
F.
Wednesday (Oct 3th): Reticulocyte counts. Click on the URL for
retic counts. Copy and read the instructions.
G. Wednesday
(Oct 10th): Manual hematocrit. Following the instructions
to access the procedure. Copy and read the instructions.
H. Wednesday
(Oct 17th): To be announced.
WORKING IN THE
LABORATORY – LAB PROTOCOL
The clinical laboratory is a speciality work area. The directions for
working in the laboratory are described in detain in the student handbook.
Other information (listed as follows) is also located in the student handbook.
01 When working in evening hours and available lockers for
CLS/CLT students.
02 Safety and security in the CNHP classroom and laboratory
buildings.
o3 Safety and health statement.
04 Helpful Learning Hints
05 Safety practices in the laboratory
06 Student code of honor
07 Program Policies: advising, attendance, faculty and
course evaluations,
grading, readmission and
retention.
08 Professional Policies: behavior, dress code,
incidents and reports, student
conduct, telephone.
09 Affective domain objectives
10 Disability services
Refer to the syllabus for CLS 2523 Hematology I for the following information:
01 Reporting the semester grade.
02 Grade retention policy.
03 Policy and code documentation.
04 Overall learning objectives
05 Critical Behavior
06 Student and Evaluation Process
07 Helpful Learning Hints
08 Confidentiality
