|
|
This web page represents a distillation of the major ideas and information associated with the Biological Science (BIOL 1003) lecture course. References to page numbers, Figures, and Tables, are associate with reading assignments Asking about Life by Tobin and Dusheck - reading assignments are noted in the Syllabus for BIOL 1003.002 for additional information. If you note any errors in the following document, I'd appreciate it if you would bring this to my attention. Email address: mhuss@astate.edu. BIOTECHNOLOGY: GENETIC ENGINEERING, GENE THERAPY, AND OTHER PRACTICAL APPLICATIONS As an understanding of the molecular basis for heredity has grown over the last century, so have the tools for manipulating genetic material and cells (e.g., DNA, RNA, cloning) in the laboratory has exploded to form the biotechnological revolution. DNA can be extracted from any organism, genes identified, their nucleotide sequences elucidated, manipulated and maneuvered into the genome (genetic make-up) of other related or non-related organisms. Restriction enzymes can act as "molecular scissors" in cutting apart DNA at specific "recognition sites" within the molecule (refer to Figure 13-5 page 282 in the textbook). Other enzymes can be used as "molecular glue" to paste different sections back together; these enzymes are called ligases - refer to Figure 13-6 page 283 in the textbook. As a result, genes harvested from one organism can be inserted into the DNA of another organism usually with the assistance of some vector (e.g., bacterial plasmid, attenuated or non-virulent virus). There are now bacteria that carry the human gene for insulin. Insulin once only harvested from animals is now harvested from bacteria capable of producing the same insulin protein produced by a human pancreas. This source of insulin is used in the treatment of diabetes. In another example, genes that result in bioluminescence in lightening bugs or fireflies has been introduced into tobacco plants to produce plants that are capable of glowing in the dark. A trait that these plants did not previously carry. The introduction of foreign DNA or genes via some mechanism into another organism is referred to as Transformation, and is a prerequisite for creating Transgenic or Genetically-Modified (GM) Organisms - refer to Figures 13-4, 13-5, 13-12, 13-13 on pages 281, 290, 291, and 293 in the textbook. Transgenic organisms are produce by: 1. Addition of new genes (to express a new gene product).
Potential Benefits of Genetic Engineering
Potential Problems of Genetic Engineering
Molecular Forensics Fragments of DNA digested with restriction enzymes can be separated through a
process of "electrophoresis" (refer to Figure 13-2 on
page 278 in the textbook) to create a "DNA fingerprint". In
humans, DNA fingerprints are unique (except between identical twins or among
identical triplets, etc.). Polymerase Chain Reaction (PCR) is used to
"amplify" or increase the amount of DNA biological samples when small amounts
are available (e.g., blood skin, hair follicles, semen, etc.);
refer to Figure 6-17 on page 179 in the textbook.
The ability to do this is especially useful in comparing biological samples left
behind at a crime scene and the opportunity for criminologists to link a suspect
to a crime by comparing their DNA fingerprint with the sample DNA.
This technique is also useful in linking the remains of unidentified human
remains with a missing or dead person. CLASSIFICATION OF LIFE'S BIODIVERSITY Over 1.5 million species of organisms have been described by biologists. Classification of life's diversity contributes to our understanding of biology. Initially, naturalist's, especially during the 18th and first part of the 19th century, were interested in classifying life's diversity in an effort to gain a broad view of the "Divine Master Plan" (see the third set of notes for further discussion of The Great Chain of Being). Carl von Linne (AKA, Carolus Linnaeus - 1707-1778) developed criteria and methods for describing and assigning species names to newly discovered group of organisms. His system of naming was called Binomial Nomenclature, two word naming system, in which each species was assigned a two word name, the genus and specific epithet. (NOTE: IN REVIEW READING MATERIAL IN THE TEXTBOOK, THE BOOK IS IN ERROR BY SAYING THAT THIS TWO WORD NAME IS COMPRISED OF A GENUS AND SPECIES NAME; THE SPECIES NAME IS ACTUALLY MADE UP OF THE GENUS AND THE SPECIFIC EPITHET - refer to Figure 19-3 on page 423 in the textbook. Examples of species include:
Note that in the previous example that the first letter of the genus name is upper case; all other letters are lower case. The species name is italicized or underlined. The author(s) name or initials may or may not appear after the species name. The author(s) are the folks who originally published a written description (in their native language and in latin). The specific epithet is often descriptive of some aspect of the species. Linnaeus and taxonomist describe a species based on one or a few species,
which are then store in a museum or herbarium collection. The specimen
used as the basis for a species description is called the type specimen.
A type specimen becomes the archetype for the entire species. Species are classified into ever broader encompassing categories. The
broadest category is the domain. There are three domains: Archaea,
Bacteria, and Eukarya - refer to Figure 1-10 on page 17
in the textbook. Members of the domains Archaea and Bacteria
are prokaryotes (refer to Figure 20-6 on page 439 in
the textbook) while members of the domain Eukarya are eukaryotes (refer
to Figure 4-5 on page 79 in the textbook). The next largest
category is the Kingdom level. Only one kingdom is found in domain Archaea,
that is, Archaebacteria. One kingdom represents the domain Bacteria, that
is, Eubacteria (the "true" bacteria - bacteria and cyanobacteria). In
prior classification schemes members of the domains Archaea and Bacteria use to
be classified together into a single kingdom called Monera. The Domain
Eukarya contains all types of eukaryotic organisms from single-celled to
multicellular forms. Four kingdoms are found in the domain Eukarya
(Kingdoms Protist, Plant, Fungi, and Animal). Of the various taxonomic categories, it is generally recognized that all levels of organizations are artificial, and the only level that is biologically-significant is the species level. FIVE OR SIX KINGDOM SYSTEM OF CLASSIFICATION --- The following table lists
characteristics common among living organisms found in the five kingdoms.
Viruses are not included in this scheme - refer to
Table 401 on page 77 in the textbook..
aAs a consumer, it acts primarily as a parasite or pathogen (disease-causing organism). bConsumers include herbivores (plant-eaters), carnivores
(meat-eaters), omnivores (mixed-diet feeders), detritivores (feeding on partly
decomposed particles of organic waste), and parasites (feeding on tissues of
living host). Classification of Life's Diversity is organized in a Hierarchical Fashion.
A mnemonic device to help remember the order of the various taxonomic categories from the broadest to the most specific would be to memorize the following statement and let the letter of each word stand for the first letter of each category. Ken's pants caught on fire, Great Scott! This hiearchial system of classification to pigeonhole species is analogous to assigning a location where you might find an individual on the planet Earth.
One final note about biological classification. The focus of modern day classification schemes is an attempt to organize biological diversity according to evolutionary relationships that exist between species. This is base on the premise that all life on this planet originated from one or a few ancestors which diverged over long periods of time to produce all the life forms we currently see on earth. Taxonomy has given way to Systematics which attempts to reconstruct genealogical relationships among all life forms - for further information review Chapter 19 in the textbook. THE MULTICELLULAR EUKARYOTES
GENERAL INTRODUCTION TO PLANTS: IT'S NOT EASY BEING GREEN Lecture Outline
1. Plants - Common examples and biodiversity. The Kingdom Plantae includes a multitude of multicellular photosynthetic autotrophs (duckweed, geranium, apple tree, oak tree, dandelion, algae, red wood tree, carrot, etc.). Lots of diversity! Plants come in different shapes, sizes. Some are short-lived, others live for hundreds of years. Plants have adapted to a wide variety of habitats, and methods of reproducing and dispersing themselves. About 248,400 species of higher plants (i.e., ferns, gymnosperms, bryophytes, flowering plants). There are about 26,900 or more species of algae. TAKE HOME MESSAGE - Many species; biodiversity is high! RELATED INTERNET LINKS: 2. Characteristics of plants
3. Plant Reproduction
4. Role of plants in the biosphere. How important are plants to other life forms on earth? Beneficial Effects of Plants
What kinds of plants/plant products have you come in contact with today? Food, medicine, spices, fibers, paper, clothing, lumber, oxygen, fuel (coal and wood), toothpicks, toilet paper, paper money, soft drinks, drugs, etc.
GENERAL INTRODUCTION TO THE KINGDOM FUNGI Lecture Outline
I. Evolution of Fungi The Kingdom Fungi is an ensemble of diverse species. Current evidence suggests that all fungal species are not derived from a single common ancestor (from within the Kingdom Protista), consequently the Fungi are polyphyletic (multiple genealogies or lineages). RELATED INTERNET LINKS:
II. Common Characteristics of Fungi
III. Human/Fungus Interactions Beneficial Effects of Fungi
Harmful Effects of Fungi
GENERAL INTRODUCTION TO THE KINGDOM ANIMALIA Lecture Outline
I. Evolution of Animals Multicellular animals appear in the fossil record in rocks dated back to about 600 million years ago. The Burgess Shale from Canada has provided a wealth of information, in addition to other sites, suggesting that animals representing most major phyla currently existing on earth and many others that went extinct underwent a "population" and "diversity" explosion during the Cambrian period (the "Cambrian Explosion"), during a relatively short period time in geological history - refer to Figure 23-2 on page 498 in the textbook. II. The Animal Body - Invertebrates and Vertebrates Strategies for making Heterotrophy in Animals Efficient
A. Invertebrates (Animals without backbones) Phylum: Porifera (sponges) - refer to Figure 23-9 on page 503 in the textbook. Water, oxygen, and food particles enter through pores and exit through a larger opening called the osculum. Sponges probably evolved independently from other animals, and in many ways are more similar to a colonial protist-like organism, composed of specialized cells that are interdependent on each other. In fact, a mashed-up live sponge, passed through a mesh, will reform into new sponges.
Phylum: Cnidaria (Hydra, jellyfish, corals, sea anemones - refer to Figures 23-10, 23-11, and 23-12 on pages 504-505 in the textbook) All forms are aquatic or marine. Two body forms exist within this group: a polyp (hydra-like or sea anemone-like) and medusa (jelly fish like). Both forms may be sedentary or free floating. These animals contain a gastrovascular cavity with a single body opening, the mouth. Tentacles are found near the entrance to the mouth. These contain nematocysts (stinging cells) that are used to paralyze prey. Phylum: Platyhelminthes (flatworms, flukes, tapeworms - refer to Figures 23-14 and 23-15 on pages 507 and 508 in the textbook) These animals exhibit bilateral symmetry and cephalization (organization of nervous and sensory systems towards one end of the body) is apparent. Phylum: Aschelminthes (roundworms, rotifers, nematodes, vinegar eels - refer to Figure 23-17on page 510 in the textbook) Example of a an Aschelminthes species that causes a human diseases: Trichinosis caused by Trichinella spiralis. Parasite enters human body through infected undercooked pork. Larvae carried by blood and lymph; these bore through vessels into muscles and organs causing damage and pain. Phylum Annelida (segmented worms - earthworms, leeches - refer to Figure 23-23 on page 516 in the textbook) Bodies divided by a series of rings. Phylum Arthropoda (insects, spiders, crabs, millipedes- refer to Figures 23-24, 23-25, and 23-26 on pages 518-520 in the textbook;) Species diversity within this phylum is high, but most share several features in common:
Phylum Mollusca (clams, snails, octopus, and squid - refer to Figures 23-18, 23-19, and 23-21 on pages 513-515 in the textbook) No exoskeleton, but some forms surrounded by a hard shell. Phylum Echinodermata (starfishes, sea urchins, sea cucumbers - refer to Figures 24-3 and 24-4 on pages 504-505 in the textbook;) Found in marine environments; animals exhibit radial symmetry; Water vascular system in starfish used to move food and gases into the body, and create suction in the suction cup like "feet" so animals can cling to rocks and gather food (e.g., opening up clams). Refer to Figures 24-1 and 24-2 on pages 524-35 in the textbook B. Vertebrates (animals with backbones - refer to Figure 24-7 on page 529 in the textbook) Phylum Chordata (fish, amphibians, reptiles, mammals, birds, etc. - refer to Figures 24-9 through 24-23 on pages 530-541 in the textbook;)
Class "The Fishes" (e.g., lampreys, cartilaginous fish, bony fish) Class Amphibia (e.g., frogs, salamanders)
Class Reptilia (e.g., snakes, turtles, lizards)
Class Aves (birds)
Class Mammalia (e.g., horses, mice, dolphins, cats, humans, etc.)
Take-home message: As various groups of animals adapted to new and changing environments and diversified, new organ systems evolved. These different organ systems make possible the various forms of animal life observed on earth today and those specimens represented in the fossil record.
Various morphological, embryological, geological (fossil record) and
molecular genetics data have been used to develop evolutionary trees or
phylogenies to reconstruct genealogical relationships among existing phyla
of animals. These vary depending on the data use in the analysis,
although some consensus does exist. RELATED INTERNET LINKS: I. Animal Tissues and Organs
Some major animal tissue types
Given time constraints, further discussion about
organs (such as the eye - see Figure 42-9 on page 873 in the textbook) and organ systems will not be discussed in further detail with the
exception of the human reproductive system. The notes and discussion
follows: II. Human Reproduction - refer to text and figures found in Chapter 43 in the textbook. The nuclei in most human cells are diploid ==> 46 chromosomes: 22 pairs of homologous or autosomal chromosomes and 1 pair of sex chromosomes. Gametogenesis - formation of haploid (23 chromosomes) gametes is the result of meiosis, cytokinesis, and cellular differentiation, which occurs in the gonads.
Hormonal Control of Human Reproduction A "releasing hormone" is produced by the hypothalamus (in the brain) causes the anterior pituitary gland to secrete luteinizing hormone (LH) and foollicle-stimulating hormone (FSH). These two hormones travel through the blood stream and migrate to the gonads.
Human Reproductive Structures and Function Male Reproductive Organs and Function Testes are found outside the abdominal cavity in a sac (scrotum).
Viable sperm are produced at a temperature lower than the human body
temperature (98.6 degrees F or 37 degrees C). Within a testis are the
seminiferous tubules (80% of testicular mass) and represents the
location of spermatogenesis. Sperm enter a coiled tubule called
the epididymis (found outside the testis). It takes about 10-12 days
for sperm to mature and gain the ability to swim. Sperm exit the
epididymis via the vas deferens; a duct that loops up behind the
bladder in the abdominal cavity and connects to the urethra.
The urethera passes through the penis. During intense sexual
arousal, blood fills blood vessels within the penis to generate an erection.
Sperm mixes with glandular secretions to produce the semen.
Semen is ejected by rhythmic contractions and leads to ejaculation.
Orgasm involves ejaculation, increases in blood pressure and heart rate,
skeletal muscles tense then relax, followed by mental relaxation and
sensations of pleasure. A refractory period during which time a second
orgasm is not possible follows. Female Reproductive Organs and Function The ovaries are the site of oogenesis (egg formation) and found within the abdominal body cavity. The uterus or womb is the location where the embryo implants and develops during pregnancy. Oviducts = Fallopian Tubes are necessary so the egg can travel from the ovary to the uterus. The vagina is opening of the body that leads to the uterus and serves as the entry point for sperm and exit point for the offspring at the end of pregnancy. Sexual union or copulation allows the semen containing sperm to enter the uterus and make its way to the egg, where fertilization is a possible outcome. Orgasm in the female leads to rhythmic contractions of the uterine muscle which may assist sperm in their journey to the egg. The ovary contains follicles (sac-like structures each containing one oocyte). Female gametes mature in a monthly cycle (approx. 28 days) called the menstrual cycle. Menstruation = 3-7 day period of blood discharge from the uterus. This bloody discharge represents the removal of the endometrium. During the course of the menstrual cycle, hormone levels rise and fall. Low levels of estrogen inhibits the release of LH and FSH, while high levels of estrogen increase the release of LH and FSH in the pituitary gland. Ovulation occurs, the egg is released starting its journey to the Fallopian tubes. The used or spent follicles fill with cells and form the corpus luteum, causing progesterone levels to rise. The increased levels of progesterone prevents additional ovulation and helps the endometrium to remain thick. Fertilization and Pregnancy One sperm unites with an egg and leads to
fertilization. The fertilized egg becomes the zygote.
Due to the union of the contents of two haploid nuclei from the sperm and
the egg, and the cytoplasm components of the egg, the zygote is diploid (23
pairs of chromosomes in the nucleus; maternal inheritance of the
organelles/cytoplasm - e.g., mitochondria). The zygote undergoes cell
division and a blastula (ball of cells) develops. As the cells
differentiate, different tissues form in the embryo. Within 7
days, the embryo reaches the uterine cavity and implants. The
placenta forms providing the embryo with oxygen and food, and generates
more progesterone, which inhibits menstruation (which would result in
a miscarriage). Gestation in humans takes 38-40 weeks; approx. 9
months after which a baby is born. Potential consequences of sexual intercourse
The following information is lacking in
your textbook, but information that is appropriate in the context of human
sexual biology and behavior.
ECOSYSTEMS AND ENERGY FLOW
- refer to text and figures in Chaper 25 found in the
textbook. Discussion in lecture as time
allows. FINAL TOPIC: HUMAN IMPACT ON THE ENVIRONMENT - refer to text and figures in Chaper 28 found in the textbook. Discussion in lecture as time allows.
This page was assembled by Martin J. Huss, who can be reached at
mhuss@astate.edu.
|
|