CURRICULUM VITAE

ANNE AURIEMMA GRIPPO

Professional Experience

2000-present Associate Professor of Biology, Arkansas State University

1995-2000 Assistant Professor of Biology, Arkansas State University

1992-1995 Instructor of Biochemistry, Department of Biochemistry & Molecular Biology, Pennsylvania State University

1987-1992 Postdoctoral Scholar, Dairy Breeding Research Center, Departments of Dairy &Animal Science and Physiology, Pennsylvania State University

1986-1987 Postdoctoral Scholar, Department of Molecular & Cell Biology, Pennsylvania State University

1984-1986 Research Assistant, Department of Medicinal Chemistry, University of North Carolina, Chapel Hill

Academic Training

1987 Ph.D. Medicinal Chemistry, University of North Carolina, Chapel Hill, NC

Dissertation Title: Sesquiterpene lactones: Mechanism of antineoplastic activity; relationship of cellular glutathione to

cytotoxicity; and disposition

1979 B.S. Chemistry; Biology minor, Fairleigh Dickinson University, Madison, NJ

Honors Research Title: Synthesis of some allenic retinoids as possible antitumor agents

Grants

2006 AR INBRE (co-I)($24,920)

2005 AR INBRE (co-I)($24,920)

2004 ASU/AR Biosciences Research Grant (co-PI) ($132,918)

2003 ASU/AR Biosciences Research Grant (co-PI) ($219,320)

2003 AR BRIN Faculty Academic Year Research Award (co-PI) ($11,330)

2002 AR BRIN Equipment Grant ($23,300)

2002 AR Biomedical Research Infrastructure Network (BRIN) Summer Research Mentoring Award (co-PI) ($13,110)

2001 NSF Chemistry Instrumentation Grant (co-PI) ($79,624)

2000 ASU Faculty Research Grant (co-PI) ($10,000)

1999 SURF-Silo Undergraduate Research Grant ($3,862)

1999 ASU College of Arts and Sciences Dean’s Research Award ($1,400)

1999 Arkansas Science and Technology Authority Basic Research Award ($26,084)

1998 Nathan Deutsch Research Award ($400)

1996 ASU Faculty Research Grant ($9,750)

Scholarly Publications

1. Grippo, AA, K Capps, B Rougeau, BJ Gurley. 2006. Analysis of flavonoid phytoestrogens in botanical and ephedra-containing dietary supplement. Ann Pharmacother. (in review)

2. Grippo, AA, B Hamilton, R Hannigan, BJ Gurley. 2006. Metal Content of Ephedra-Containing Dietary Supplements and other Select Botanicals. Am J Health-Syst Pharm. 63:635-644.

3. Grippo, AA, Y Luo, B Rougeau WV Wyatt. 2000. HPLC analysis of mono-saccharides in whole and regional, staged bovine oviductal fluid. Theriogenology. 35:717-721.

4. Grippo, AA, Y Xie, B Rougeau WV Wyatt. 1999. Analysis of phytoestrogens by high pressure liquid chromatography. J. AR Acad. Sci. 53:61-66.

5. Grippo, AA, AL Way G.J Killian. 1995. Effect of bovine ampullary and isthmic oviductal fluid on motility, acrosome reaction and fertility of bull spermatozoa. J. Reprod. Fertil. 105:57-64.

6. Grippo, AA, SH Anderson, M.A Henault, G.JKillian. 1994. Cholesterol, phospholipid and phosphor-lipase activity of ampullary and isthmic fluid from the bovine oviduct. J. Reprod. Fertil. 102:87-93.

7. Grippo, AA, M.A Henault, SH Anderson, GJ Killian. 1992. Cation concentrations in fluid from the oviduct ampulla and isthmus of cows during the estrous cycle. J. Dairy Sci. 75:58-65.

8. Kavanaugh, JF, AA Grippo, GJ Killian. 1992. Cannulation of the bovine ampullary and isthmic oviduct. J. Invest. Surg. 5:11-17.

9. Grippo, AA, IH Hall, H Kiyokawa, O Muraoka, Y-C Shen, K-H Lee. 1992. The cytotoxicity of helenalin, its mono and difunctional esters, and related sesquiterpene lactones in murine and human tumor cells. Drug Design Disc. 8:191-206.

10. Grippo, AA, SD Wyrick, KH Lee, RP Shrewsbury, IH Hall. 1991. Disposition of an antineoplastic sesquiterpene lactone, [3H]-plenolin, in BDF1 mice. Pl. Med. 7:299-314.

11. Hall, IH, AA Grippo, DJ Holbrook, JJ Chang, LM Yang, SG Chaney, K.H Lee. 1990. Renal, hepatic, cardiac and thymic acute toxicity afforded by bis(helenalinyl) malonate in BDF1 mice. Toxicology 65:205-216.

12. Hall, IH, AA Grippo, DJ Holrook, GB Roberts, HC.Lin, HL Kim, KH Lee. 1989. Role of thiol agents in protecting against the toxicity of helenalin in tumor-bearing mice. Planta Med. 5:513-517.

13. Wedler, F. C., B. W. Ley and A. A. Grippo. 1989. Manganese (II) dynamics and distribution in glial cells cultured from chick cerebral cortex. Neurochem. Res. 14:1129-1135.

14. Tholey, G, M Ledig, P Kopp, L Sargentini-Maier, M Leroy, AA Grippo , FC Wedler. 1988. Levels and subcellular distribution of physiologically important metal ions in neuronal cells cultured from chick embryo cerebral cortex. Neurochem. Res. 13:1163-1167.

15. Tholey, G, M.Ledig, P Mandel, L Sargentini, AH Frivole, M Leroy, AA Grippo , FC Wedler. 1988. Concentrations of physiologically important metal ions in glial cells cultured from chick cerebral cortex. Neurochem. Res. 13:45-50.

16. Williams, W. L., I. H. Hall, A. A. Grippo, C. B. Oswald, K. H. Lee, D. Holbrook and S. Chaney. 1988. Inhibition of nucleic acid synthesis in P-388 lymphocytic leukemia tumor cells by helenalin and bis(helenalinyl) malonate in vivo. J. Pharm. Sci. 77:178-184.

17. Chapman, DE, GB Roberts, DJ Reynolds, AA Grippo, DJ Holbrook, IH Hall, SG Chaney, J Chang, K. H. Lee. 1988. Acute toxicity of helenalin in BDF1 mice. Fund. App. Toxicology 10:302-312.

18. Hall, I. H., W. L. Williams, Jr., A. A. Grippo, K.-H. Lee, D. J. Holbrook and S. G. Chaney. 1988. Inhibition of nucleic acid synthesis in P-388 lymphocytic leukemia cells in culture by sesquiterpene lactones. Anticancer Res. 8:33-42.

19. Wyrick, S. D., F. T. Smith, W. E. Kemp and A. A. Grippo. 1987. Effects of [(N-alkyl-1H,3H-isoindolin-1,3-dione-5-yl)oxy]-alkanoic acids, [(N-alkyl-1H,3H-isoindolin-1-one-5-yl)oxy]-butanoic acids and related derivatives on chloride influx in primary astroglial cultures. J. Medicinal Chem. 30:1798-1806.

20. Hall, I. H., A. A. Grippo, K.-H. Lee, S. G. Chaney and D. J. Holbrook. 1987. Effect of helenalin and bis(helenalinyl) malonate on nucleic acid and protein synthesis in human KB carcinoma cells. Pharm. Res. 4:509-515.

Presentations

1. AA Grippo, C Brown, S Trauth, R Grippo, B Gurley. Metabolife and Its Constituents Increase Atypical Cardiac Cells, An Effect Exacerbated by Nicotine. Soc. Toxicol. (2006)

2. B Gurley, SF Gardner, K Williams, A Grippo, Hemodynamic and electrocardiographic effects of an “Ephedra-free” dietary supplement (Metabolife Ultra^TM). Am. Soc. Clin. Phamacol. Ther. (2006)

3. C Brown, P MacFarland, S Trauth, R Grippo, A Grippo, Cardiovascular effects of combined

4. AA Grippo, S Snipes, R Raeven, W Kennedy, W Humphrey. Genistein effects on sheep reproduction. Invited lecture: So. Assoc. Agric. Sci. (2005)

5. A.A. Grippo, A. A. Stokes, C. E. Brown, L. Rosa, M. Mary, R. S. Grippo, S. Trauth, B. J. Gurley. Does ephedra alone effect rat cardiovascular physiology and tissue morphology differently than ephedra contained in Metabolife? AR Biotech. Inst. Symposium (2004).

6. A.A. Grippo, S. Snipes, A. Davis, W. Humphrey. Detection of genistein in serum and oviductal fluid of ewes administered Biochanin A. Biol. Reprod. 64 (Suppl. 1):89 (2004).

7. AA Grippo. Studies of the effects of phytoestrogens on mammalian reproduction. Invited lecture: University of Memphis Biology Seminar Series (2003).

8. K Capps, B Rougeau, B Gurley, A Grippo. Bioflavonoids in herbal supplements. Southern Regional Discussion Group, Amer. Assoc. Pharm. Sci. (2003).

9. BT Hamilton, RE Hannigan, BJ Gurley, AA Grippo. Metals concentrations in over-the-counter herbal preparations. Southern Regional Discussion Group, Amer. Assoc. Pharm. Sci. (2003).

10. A. A. Grippo, K. Capps, B. J. Gurley. Bioflavonoids in herbal supplements. AR Biotech. Inst. Symposium (2002).

11. AA Grippo. Reproductive toxicology of phytoestrogens. Invited lecture: UC Davis Toxicology Seminar Series (2001).

12. WD Bryant, W Humphrey, AA Grippo. The effect of estrogenic agents on ram sperm acrosome reaction. 21^st Annual Meeting, Soc. Env. Tox. & Chem. (2000).

13. Y Luo, B Rougeau, W Wyatt, A Grippo. Availability of monosaccharides in whole and regional, staged bovine oviductal fluid. Biol. Reprod. 60 (Suppl. 1):158 (1999).

14. A. A. Grippo, Y. Xie, B. Rougeau, W. V. Wyatt. Analysis of phytoestrogens by high pressure liquid chromatography. Proc. AR Acad Sci.: 92 (1999)

15. W. Hayes, J. Pierce, J. Farris, A. Grippo. Vitellogenin purification from the slider turtle Trachemys scripta. Proc. AR Acad. Sci.:18 (1998)

16. A. Strecker, A. Grippo. Displacement of steroid hormones from sex hormone binding globulin by phytoestrogens. Proc. AR Acad. Sci.:36 (1998)

17. E. Winchester, J. Farris, A. Grippo, R. Grippo. Effects of aldicarb on cholinesterase activity of two freshwater molluscs (Ligumia subrostrata and Corbicula fluminea). Soc. Env. Tox. & Chem. (1997)

18. G. J. Killian, A. A. Grippo. Incubation in ampullary oviductal fluid improves the ability of sperm to fertilize bovine oocytes in vitro. Proc. of the 12th Int. Congress on Animal Repro. 2:650-652 (1992)

19. M. A. Henault, A. L. Way, A. A. Grippo, G. J. Killian. A heterospermic, competitive penetration assay utilizing zona-free bovine oocytes. Biol. of Reprod. 46(Suppl. 1):74 (1992)

20. R. S. King, A. A. Grippo, G. J. Killian. Bovine estrus-associated protein stimulates capacitation of bull sperm. Biol. of Reprod. 44(Suppl. 1):135 (1991)

21. A. A. Grippo, D. A. Chapman, G. J. Killian. An improved assay for phospholipase A2 activity in sperm. J. Andrology 10:47 (1989)

22. S. D. Wyrick, F. T. Smith, A. A. Grippo. Synthesis of agents affecting astrocytic chloride influx. Proc. of the Meeting in Miniature, ACS Research Triangle Section (1986)

23. D. E. Chapman, G. B. Roberts, A. A. Grippo, I. H. Hall, S. G. Channey, J. Chang, D. J. Reynolds, D. J. Holbrook. Acute toxicity of helenalin in BDF1 mice. The Toxicologist. 5:299 (1986)

24. A. A. Grippo, S. D. Wyrick, R. P. Shrewsbury, S. G. Chaney, D. J. Holbrook, K. H. Lee, I. H. Hall. The disposition of [3H]-11,13-dihydrohelenalin, a derivative of the sesquiterpene lactone helenalin. The Pharmacologist. 27:109 (1985)

25. A. A. Grippo, W. Williams, K. H. Lee, S. Chaney, D. Holbrook, I. H. Hall. Antineoplastic activity of the natural product helenalin. Proc. of the International Res. Congress on Natural Products (1985)

26. A. A. Grippo, I. H. Hall, D. J. Holbrook, S. G. Chaney. The role of cellular levels of reduced glutathione on the efficacy of an antitumor sesquiterpene lactone. Proc. of the ACS Southeastern Regional Meeting (1984)

27. A. A. Grippo, I. H. Hall, K. H. Lee, D. J. Holbrook. The toxicology and mechanism of helenalin, an antitumor sesquiterpene lactone. Proc. of the ACS Southeastern Regional Meeting (1983)

28. J. R. Dyer, D. R. King, A. M. Auriemma. Studies of the reaction between carbon disulfide and sodium hydroxide. Proc. of the ACS Northeastern Regional Meeting (1979)